TransScript一步法去除 gDNA及第一链 cDNA 合成试剂盒-AT311
产品名称
TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix (AT311)
应用专利
专利号：ZL201710031139.6
高分文献集锦
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•Wang S, Du Y, Zhang B, et al. Transplantation of chemically induced pluripotent stem-cell-derived islets under abdominal anterior rectus sheath in a type 1 diabetes patient[J]. Cell, 2024.(IF 45.50)
•Zhao P Z, Yang H, Sun J Y, et al. Targeted MYC2 stabilization confers citrus Huanglongbing resistance [J]. Science, 2025.(IF 44.70)
•Chen J, Ou Y, Luo R, et al. SAR1B senses leucine levels to regulate mTORC1 signalling[J]. Nature, 2021.(IF 42.77)
•Chen J, Ou Y, Yang Y, et al. KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing[J]. Nature, 2018.(IF 40.13)
•Zhao K, Xue H, Li G, et al. Pangenome analysis reveals structural variation associated with seed size and weight traits in peanut[J]. Nature genetics, 2025.(IF 31.80)
•Liu S, Liu C, Lv X, et al. The chemokine CCL1 triggers an AMFR-SPRY1 pathway that promotes differentiation of lung fibroblasts into myofibroblasts and drives pulmonary fibrosis[J]. Immunity, 2021.(IF 31.74)
•Huang L, Wei M, Li H, et al. GP73-dependent regulation of exosome biogenesis promotes colorectal cancer liver metastasis[J]. Molecular Cancer, 2025.(IF 27.70)
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•Zhu J, Zhong X, He H, et al. Generation of human expandable limb-bud-like progenitors via chemically induced dedifferentiation[J]. Cell Stem Cell, 2024.(IF 19.80)
•Wang R, Xue Y, Fan J, et al. A systems genetics approach reveals PbrNSC as a regulator of lignin and cellulose biosynthesis in stone cells of pear fruit[J]. Genome Biology, 2021.(IF 17.90)
•Zhang Y, Chen W, Wu D, et al. Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli[J]. Nucleic acids research, 2022.(IF 16.97)
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•Zhao M, Wang B, Zhang C, et al. The DJ1-Nrf2-STING axis mediates the neuroprotective effects of Withaferin A in Parkinson's disease[J]. Cell Death & Differentiation, 2021.(IF 15.82)
•Liu J C, Li L, Yan H C, et al. Identification of oxidative stress–related Xdh gene as a di (2‐ethylhexyl) phthalate (DEHP) target and the use of melatonin to alleviate the DEHP‐induced impairments in newborn mouse ovaries[J]. Journal of pineal research, 2019.(IF 15.22)
•Zhang C, Wang Y, Zhen Z, et al. mTORC1 Mediates Biphasic Mechano‐Response to Orchestrate Adhesion‐Dependent Cell Growth and Anoikis Resistance[J]. Advanced Science, 2023.(IF 15.10)
•Wang Y, Wang Z, Chen W, et al. A KDPG sensor RccR governs Pseudomonas aeruginosa carbon metabolism and aminoglycoside antibiotic tolerance[J]. Nucleic Acids Research, 2023.(IF 14.90)
产品特点
• 在同一反应体系中，同时完成反转录与基因组 DNA 的去除，操作简便，降低污染机率。
• 产物用于 qPCR：反转录 15 分钟；产物用于 PCR：反转录 30 分钟。
• 反应结束后，同时热失活 RT/RI 与 gDNA Remover。
• 合成片段 ≤12 kb。
数据展示
PCR 检测
qPCR 检测
以人、烟草 RNA 为模板，分别使用 TransGen 和 Company V 产品进行反转录，PCR (AS131) 和 qPCR (AQ601) 检测反转录效果。结果表明， TransGen 产品反转录效果更好。
产品信息
