支原体检测试剂盒 (PCR 法) - FM311

  产品名称  

TransDetect® PCR Mycoplasma Detection Kit(FM311)

  高分文献集锦  

•Lei Z, Meng H, Liu L, et al. Mitochondrial base editor induces substantial nuclear off-target mutations[J]. Nature, 2022.(IF 69.50)

•Huang J, Lin Q, Fei H, et al. Discovery of deaminase functions by structure-based protein clustering[J]. Cell, 2023.(IF 65.00)

•Liu J L, Yan X Q, Wu H, et al. RNA codon expansion via programmable pseudouridine editing and decoding[J]. Nature, 2025.(IF 50.50)

•Xu D, Zhao H, Jin M, et al. Modulating TRADD to restore cellular homeostasis and inhibit apoptosis[J]. Nature, 2020.(IF 42.78)

•Fei H Y, LI Y J, Liu Y J, et al. Advancing protein evolution with inverse folding models integrating structural and evolutionary constraints[J]. Cell, 2025.(IF 42.50)

•Sun C, Li H C, Liu Y J, et al. Iterative recombinase technologies for efficient and precise genome engineering across kilobase to megabase scales[J]. Cell, 2025.(IF 42.50)

•Bai X, Sun P, Wang X, et al. Structure and dynamics of the EGFR/HER2 heterodimer[J]. Cell Discovery, 2023.(IF 38.07)

•Wang D, Li X, Jiao D, et al. LCN2 secreted by tissue-infiltrating neutrophils induces the ferroptosis and wasting of adipose and muscle tissues in lung cancer cachexia[J]. Journal of Hematology & Oncology, 2023.(IF 28.50)

•Nian Z, Dou Y, Shen Y, et al. Interleukin-34-orchestrated tumor-associated macrophage reprogramming is required for tumor immune escape driven by p53 inactivation[J]. Immunity.2024.(IF 25.50)

•Li Z, Wang Z, Zhong C, et al. P53 upregulation by USP7-engaging molecular glues[J]. Science Bulletin, 2024.(IF 18.80)

•Xu J, Liang Y, Li N, et al. Clathrin-associated carriers enable recycling through a kiss-and-run mechanism[J]. Nature Cell Biology, 2024. (IF 17.30)

•Fan S, Qi M, Qi Q, et al. Targeting FAPα-positive lymph node metastatic tumor cells suppresses colorectal cancer metastasis[J]. Acta Pharmaceutica Sinica B, 2024.(IF 14.80)

•Wang J, An Z, Wu Z, et al. Spatial organization of PI3K-PI (3, 4, 5) P3-AKT signaling by focal adhesions[J]. Molecular Cell, 2024.(IF 14.50)

•Zhang W, Yin C, Qi L, et al. RFWD3 reprograms nucleotide metabolism through PHGDH to induce chemoresistance in osteosarcoma[J]. Advanced Science, 2025.(IF 14.10)

•Nian Z, Zheng X, Dou Y, et al. Rapamycin pretreatment rescues the bone marrow AML cell elimination capacity of CAR-T cells[J]. Clinical Cancer Research, 2021.(IF 12.50)

•Wang L, Wang Y, He X, et al. CD70-targeted iPSC-derived CAR-NK cells display potent function against tumors and alloreactive T cells[J]. Cell Reports Medicine, 2025.(IF 10.60)

•Gu Y, Wei X, Sun Y, et al. miR-192-5p silencing by genetic aberrations is a key event in hepatocellular carcinomas with cancer stem cell features[J]. Cancer research, 2019.(IF 8.38)

•Liu Z, Chen Z, Wang J, et al. Mouse avatar models of esophageal squamous cell carcinoma proved the potential for EGFR-TKI afatinib and uncovered Src family kinases involved in acquired resistance[J]. Journal of Hematology & Oncology, 2018.(IF 7.33)

•Lu B, Dong L, Yi D, et al. Transposase-assisted tagmentation of RNA/DNA hybrid duplexes[J]. Elife, 2020.(IF 7.08)

  产品特点  

• 灵敏度高:优化的高敏检测体系,最大程度的降低漏检风险。

• 覆盖全面:针对支原体 16S rRNA 序列设计多条特异性引物,可全面覆盖包括实验室常见支原体污染类型在内的 54 种支原体。

• 简便快捷:无需复杂前处理,65 min 完成扩增,较 V1 版本扩增时间缩短 30 min。

• 稳定性好:耐受 5 μl 不同类型的大体积样本,热加速稳定性好。

• 特异性高:与常见细菌及工程细胞无交叉扩增,结果可靠。

  数据展示  

覆盖度好

覆盖度好

分别使用 TransGen 和 Company G 产品对 7 种常见支原体标准品进行检测,结果表明, TransGen 产品均能检出,而 Company G 扩增能力较弱。

灵敏度高

灵敏度高

分别使用 TransGen 和 Company G 产品检测 100 copies/μl 的支原体标准品,结果表明,TransGen 产品的检测灵敏度可达 100 copies/μl,而 Company G 未成功检出。

抗抑制性强

抗抑制性强

分别使用 TransGen 和 Company G 产品扩增 1-5 μl 的支原体阳性细胞培养液,结果表明,TransGen 产品抗抑制性好,5 μl 上样体积仍能有效扩增。

可检测 54 种支原体

可检测 54 种支原体

  产品信息  

产品信息

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