基础版同源重组无缝克隆试剂盒-CU201
产品名称
pEASY®-Basic Seamless Cloning and Assembly Kit (CU201)
高分文献集锦
•You L, Omollo E O, Yu C, et al. Structural basis for intrinsic transcription termination[J]. Nature, 2023.(IF 69.50)
•Huang J, Yang L, Yang L, et al. Stigma receptors control intraspecies and interspecies barriers in Brassicaceae[J]. Nature, 2023.(IF 64.80)
•Liu R, Yang J, Yao J, et al. Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins[J]. Nature biotechnology, 2022.(IF 54.90)
•Jiang L, Xie X, Su N, et al. Large Stokes shift fluorescent RNAs for dual-emission fluorescence and bioluminescence imaging in live cells[J]. Nature Methods, 2023.(IF 48.00)
•Wu M, Xu G, Han C, et al. lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription[J]. Science, 2021.(IF 47.73)
•Zhang R, He Z, Shi Y, et al. Amplification editing enables efficient and precise duplication of DNA from short sequence to megabase and chromosomal scale[J]. Cell, 2024.(IF 45.50)
•Bai X, Sun P, Wang X, et al. Structure and dynamics of the EGFR/HER2 heterodimer[J]. Cell Discovery, 2023.(IF 33.50)
•Lei X, Huang A, Chen D, et al. Rapid generation of long, chemically modified pegRNAs for prime editing[J]. Nature Biotechnology, 2024.(IF 33.10)
•Zou Z P, Du Y, Fang T T, et al. Biomarker-responsive engineered probiotic diagnoses, records, and ameliorates inflammatory bowel disease in mice[J]. Cell Host & Microbe, 2022.(IF 31.32)
•Zhang Y, Dai F, Chen N, et al. Structural insights into VAChT neurotransmitter recognition and inhibition[J]. Cell Research, 2024.(IF 28.10)
•Yan X, Li Y, Wang G, et al. Molecular basis for recognition of Gly/N-degrons by CRL2ZYG11B and CRL2ZER1[J]. Molecular Cell, 2021.(IF 17.97)
•Chen Q, Hu T, Li X, et al. Phosphorylation of SWEET sucrose transporters regulates plant root: shoot ratio under drought[J]. Nature plants, 2022,(IF 17.35)
•Jiang C, Yu C, Sun S, et al. A new anti-CRISPR gene promotes the spread of drug-resistance plasmids in Klebsiella pneumoniae[J]. Nucleic Acids Research, 2024.(IF 16.60)
•Wan W, Dong H, Lai D H, et al. The Toxoplasma micropore mediates endocytosis for selective nutrient salvage from host cell compartments[J]. Nature communications, 2023.(IF 16.60)
•Ye D, Shao Y, Li W R, et al. Characterization and Engineering of Two Highly Paralogous Sesquiterpene Synthases Reveal a Regioselective Reprotonation Switch[J]. Angewandte Chemie International Edition, 2024.(IF 16.60)
•Zhang H W, Huang K, Gu Z X, et al. A cryo-EM structure of KTF1-bound polymerase V transcription elongation complex[J]. Nature Communications, 2023.(IF 16.60)
•Wang T, Zou J, Wang K, et al. Chemoenzymatic Synthesis of the Cyclopiane Family of Diterpenoid Natural Products[J]. Angewandte Chemie International Edition, 2025(IF 16.10)
•Chen Q, Hu T, Li X, et al. Phosphorylation of SWEET sucrose transporters regulates plant root: shoot ratio under drought[J]. Nature plants, 2022.(IF 15.79)
•Chen D, Song Z, Han J, et al. Targeted Discovery of Glycosylated Natural Products by Tailoring Enzyme-Guided Genome Mining and MS-Based Metabolome Analysis[J]. Journal of the American Chemical Society, 2024.(IF 15.00)
产品特点
• 快速：仅需要 5-15 分钟反应时间。
• 支持多片段连接：最高可实现 5 个片段的无缝连接。
• 简单：不受片段酶切位点的影响，无需对片段酶切。
• 高效：阳性率达 95% 以上。
• 提供在线引物设计软件：智能在线工具，快速生成高特异性引物。
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组装效率高
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产品信息
