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通用版同源重组无缝克隆试剂盒-CU101

产品名称

pEASY^®-Uni Seamless Cloning and Assembly Kit (CU101)

高分文献集锦

•Xu Y, Zhu T F. Mirror-image T7 transcription of chirally inverted ribosomal and functional RNAs[J]. Science, 2022.(IF 63.71)

•Shi J, Mei C, Ge F, et al. Resistance to Striga parasitism through reduction of strigolactone exudation[J]. Cell, 2025.(IF 42.50)

•Bai X, Sun P, Wang X, et al. Structure and dynamics of the EGFR/HER2 heterodimer[J]. Cell Discovery, 2023.(IF 38.07)

•Wang H, Yang J, Cai Y, et al. Macrophages suppress cardiac reprogramming of fibroblasts in vivo via IFN-mediated intercellular self-stimulating circuit[J]. Protein & Cell, 2024.(IF 21.10)

•Xu J, Liang Y, Li N, et al. Clathrin-associated carriers enable recycling through a kiss-and-run mechanism[J]. Nature Cell Biology, 2024.(IF 17.30)

•Li B, Zhu L, Lu C, et al. circNDUFB2 inhibits non-small cell lung cancer progression via destabilizing IGF2BPs and activating anti-tumor immunity[J]. Nature communications, 2021.(IF 14.92)

•Shi C, Yang X, Hou Y, et al. USP15 promotes cGAS activation through deubiquitylation and liquid condensation[J]. Nucleic Acids Research, 2022.(IF 14.90)

•Wang J, An Z, Wu Z, et al. Spatial organization of PI3K-PI (3, 4, 5) P3-AKT signaling by focal adhesions[J]. Molecular Cell, 2024.(IF 14.50)

•Liu S, Fan L, Liu Z, et al. A Pd1–Ps–P1 feedback loop controls pubescence density in soybean[J]. Molecular plant, 2020.(IF 12.08)

•Jin Q, Yang X, Gou S, et al. Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression[J]. Science China Life Sciences, 2022.(IF 10.37)

•Mu S, Chen H, Li Q, et al. Enhancing prime editor flexibility with coiled-coil heterodimers[J]. Genome Biology, 2024.(IF 10.10)

•Du G, Xiong L, Li X, et al. Peroxisome elevation induces stem cell differentiation and intestinal epithelial repair[J]. Developmental cell, 2020. (IF 10.09)

•Tang Y, Gao C C, Gao Y, et al. OsNSUN2-mediated 5-methylcytosine mRNA modification enhances rice adaptation to high temperature[J]. Developmental cell, 2020.(IF 10.09)

•Li J, Bai Y, Liu Y, et al. Transcriptome-based chemical screens identify CDK8 as a common barrier in multiple cell reprogramming systems[J]. Cell Reports, 2023.(IF 8.80)

•Que Y, Shu X, Wang L, et al. Inactivation of SERCA2 Cys674 accelerates aortic aneurysms by suppressing PPARγ[J]. British Journal of Pharmacology, 2021.(IF 8.74)

•Wang Y, Dong H, Qu Y, et al. Circabidian rhythm of sex pheromone reception in a scarab beetle[J]. Current Biology, 2024.(IF 8.10)

•Sun S, Liu D, Luo W, et al. Domestication-selected COG4-OsbZIP23 module regulates chilling tolerance in rice[J]. Cell Reports, 2024.(IF 7.50)

•Du J, Huang Z, Li Y, et al. Copper exerts cytotoxicity through inhibition of iron-sulfur cluster biogenesis on ISCA1/ISCA2/ISCU assembly proteins[J]. Free Radical Biology and Medicine, 2023.(IF 7.40)

•Zhu L, Li B, Li R, et al. METTL3 suppresses pancreatic ductal adenocarcinoma progression through activating endogenous dsRNA-induced anti-tumor immunity[J]. Cellular Oncology, 2023.(IF 6.60)

•Song N, Chen L, Ren X, et al. N-Glycans and sulfated glycosaminoglycans contribute to the action of diverse Tc toxins on mammalian cells[J]. PLoS Pathogens, 2021.(IF 6.20)

产品特点

• 高效连接：阳性率高，克隆数多。

• 支持多片段连接：最高可实现 15 个片段无缝连接。

• 快速重组：5-15 min 即可完成反应。

• 大容量组装：可成功构建 31.8 kb 的质粒 ( 载体 + 片段 )。

• 长载体组装：支持 14 kb 长载体组装。

• 广谱兼容：支持低浓度 (0.003 pmol) 单片段、多片段高效连接。

• 提供在线引物设计软件：智能在线工具，快速生成高特异性引物。

数据展示

组装效率高